The mice are maintained under Specific Pathogen Free conditions
using autoclaved static caging systems. Rodents are permitted
entry into the facility/room after an 8 week quarantine period.
The health status of all the colonies are monitored every
other month by exposing sentinel cages containing Tac: (SW)fBR
female mice to solied bedding. Each holding room contains
at least 2 sentinel cages (1 sentinel per 200 resident cages).
One mouse per sentinel cage is evaluated during each testing
cycles as indicated below:
There are three separate testing cycles: every other month;
semiannual; and annual. The agents screened at each interval
are as follows:
| Every other month |
Semiannual |
Annual |
SEND
|
Every other month testing including: |
Semiannual testing including: |
PVM
|
LCMV |
MTLV |
| MHV |
MAV |
MCMV |
| MVM |
ECTRO |
Hantaan |
| MPV-1 |
K |
E. cuniculi |
| MPV-2 |
POLY |
CAR bacillus |
| NS-1 |
Cecal/duodenal exam for protozoa |
|
| GDVII |
Cecal culture
for
Salmonella sp. and C. rodentium (pooled) |
|
| REO |
Aerobic respiratory tract culture (pooled) |
|
| EDIM |
Cecal and colon for helminthes |
|
| MPVL |
Exam of colon for Helicobacter sp.,
H.hepaticus and H.bilis |
|
| MNV |
Gross pathology; histology if needed. |
|
| Pooled Fecal Float |
Fecal Exam for Helicobacter sp.,
H.hepaticus and H.bilis |
|
| Anal Tape Test |
|
|
| Helicobacter Testing as needed |
|
|
