The mice are maintained under Specific Pathogen Free conditions
using ventilated isolator caging systems. Rodents are permitted
entry into the barrier via an approved vendor or through the
rederivation program. The health status of all the colonies
are monitored monthly by exposing sentinel cages containing
Tac: (SW)fBR female mice to solied bedding. Each holding room
contains at least 1 sentinel cages (1 sentinel per 50 resident
cages). One mouse per sentinel cage is evaluated during each
testing cycles as indicated below:
There are three separate testing cycles: monthly; semiannual;
and annual. The agents screened at each interval are as follows:
| Monthly |
Semiannual |
Annual |
SEND
|
Monthly testing including: |
Semiannual testing including: |
PVM
|
LCMV |
MTLV |
| MHV |
MAV |
MCMV |
| MVM |
ECTRO |
Hantaan |
| MPV-1 |
K |
E. cuniculi |
| MPV-2 |
POLY |
CAR bacillus |
| NS-1 |
Cecal/duodenal exam for protozoa |
|
| GDVII |
Cecal culture
for
Salmonella sp. and C. rodentium (pooled) |
|
| REO |
Aerobic respiratory tract culture (pooled) |
|
| EDIM |
Cecal and colon for helminthes |
|
| MPUL |
Exam of colon for Helicobacter sp.,
H.hepaticus and H.bilis |
|
| MNV |
|
|
| Pooled Fecal Float |
Gross pathology; histology if needed. |
|
| Anal Tape Test |
Fecal Exam for Helicobacter sp.,
H.hepaticus and H.bilis |
|
| Helicobacter Testing as needed |
|
|
